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KMID : 0383819740210030149
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Tuberculosis and Respiratory Diseases
1974 Volume.21 No. 3 p.149 ~ p.155
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An Experimental Study of Direct Smear Examinations for Detecting Patients with Pulmonary Tuberculosis
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김규태(金奎泰)/Kyu-Tae Kim
김성진(金成鎭)/안성규(安聖奎)/Sung-Chin Kim/Sung-kyu Ahn
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Abstract
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Evaluations of three various techniques of smear preparation were made. Also the
results of microscopic reading at various numbers of fields were compared to obtain an
optimal number of fields for effective reading.
These procedures were studied since they are basic diagnostic tools in tuberculosis
laboratory work.
1) Smear Preparations by the wire-loop method(WLS), the wooden-applicator
method(WAS) and the slide press-down method, were thoroughly compared to evaluate.
i. Safety during work
ii. Simplicity (Convenience) and
iii. Discovery rates
2) From 50 up to 500 microscopic fields were read on negative slides and one-plus
positive slides to obtain discovery rates.
In the cafe of one-plus slides, the results of the 150 field-reading were employed as a
obtain the cumulative number of bacilli by the criterion to number of microscopic fields
increased.
Results obtained in the study were as follows.
1. Of the three methods concerned in the smear preparation study, both the wire-loop
method and the wooden-applicator method(with a few drawbacks) were found to have
yielded the best results in many respects, whereas the slide press-down method showed
less accurate results as well as even some health safely risks in the smear preparation.
The slide press-down method showed some unfavourable responses from microscopists
concerning its accuracy and also most felt that the procedure was unsafe and not
widely practiced in this country.
2. On the basis of the results obtained from the prolonged microscopy on smears, the
300-field reading yielded the appearance of nearly twice as many tubercle bacilli as the
150-field reading. Therefore, smears considered presumably negative must be read at
more than 300 microscopic-fields in the tuberculosis laboratories.
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